Harnessing Peptide Binding to Capture and Reclaim Phosphate

Harnessing Power to Capture Leadership

Application of FITC for detecting the binding of antiangiogenic peptide to HUVECs

Angiogenesis is the generation of new blood vessels from the existing vasculature. The angiogenic programme requires the degradation of the basement membrane, endothelial cell migration and invasion of the extracellular matrix, with endothelial cell proliferation and capillary lumen formation before maturation and stabilization of the new vasculature. Angiogenesis is dependent on a delicate equ...

Using a membrane bioreactor to reclaim wastewater

eclaimed municipal wastewater is becoming a source of public water supplies in places where water is limited. In countries such as Japan, South Africa, and France, legislation encouraging innovative technologies that render wastewater fit for reuse has stimulated the development of alternative processes.1 The use of membranes in tertiary treatment and advanced purification evolved as a promisin...

Phosphate Binding to Alkaline Phosphatase

The metal ion dependence of a2P binding to Escherichia coli alkaline phosphatase has been studied by means of equilibrium dialysis. Whereas the apoenzyme does not bind phosphate (no sites with K < 5 X 10-5 M), the addition of 2 Zn(I1) cations per molecule induces the tight binding of 1 phosphate dianion (K = 6 X 1OF M). The magnitude of this binding constant is affected by ionic strength, pH, a...

Harnessing supramolecular peptide nanotechnology in biomedical applications

The harnessing of peptides in biomedical applications is a recent hot topic. This arises mainly from the general biocompatibility of peptides, as well as from the ease of tunability of peptide structure to engineer desired properties. The ease of progression from laboratory testing to clinical trials is evident from the plethora of examples available. In this review, we compare and contrast how...